发货周期:
干冰冻存管运输3天(1x106cells/冻存管x2)
常温运温1-2周发货 (1x106 cells/T25培养瓶)
KYSE-150来源于一位49岁日本女性患者的中段食管原发性鳞状细胞癌 (ESCC)组织,保留了原癌组织的关键生物学特征,如鳞状上皮分化特征、恶性增殖能力等,广泛用于食管癌发病机制研究、抗癌药物筛选与药效评价、肿瘤异质性研究
细胞名称:人食管鳞癌细胞(经抗食管鳞状细胞癌药物通路验证)
细胞简称:KYSE-150
产品货号:TCH-C236-UD
种属来源:人
组织来源:食管
疾病特征:食管鳞癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:RPMI-1640 +10% FBS+1% P/S
配套培养基货号:TCH-G236
传代比例:1:2-1:4,每2-3天换液一次
倍增时间:24-48 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Kanda, Y., Nishiyama, Y., Shimada, Y., Imamura, M., Nomura, H., Hiai, H., Fukumoto, M. (1994). Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. International journal of cancer.Journal international du cancer 58 ( 2 ): 291-297 . Http://www.ncbi.nlm.nih.gov/pubmed/7913084 Shimada, Y., Imamura, M., Wagata, T., Yamaguchi, N., Tobe, T. (1992). Characterization of 21 newly established esophageal cancer cell lines. Cancer 69 ( 2 ): 277-284 . Http://www.ncbi.nlm.nih.gov/pubmed/1728357 Shimada, Y., Imamura, M. (1993). Prognostic significance of cell culture in carcinoma of the oesophagus. The British journal of surgery 80 ( 5 ): 605-607 . Http://www.ncbi.nlm.nih.gov/pubmed/8518899 Tanaka, H., Shibagaki, I., Shimada, Y., Wagata, T., Imamura, M., Ishizaki, K. (1996). Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. International journal of cancer.Journal international du cancer 65 ( 3 ): 372-376 . Http://www.ncbi.nlm.nih.gov/pubmed/8575860
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收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。