HyCyte® | 海星生物

产品名称：U-2 OS细胞Luc基因过表达稳转细胞株

产品货号：TCH-C365L

转导方法：慢病毒法

抗性基因：Puro

荧光基因：Luciferase

克隆类型：Poolcells

靶细胞名称：人骨肉瘤细胞

靶细胞简称：U-2 OS

种属来源：人

组织来源：骨

疾病特征：骨肉瘤

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：McCoy's 5A+10%FBS（胎牛血清）+2.0 ug/mL Puromycin+1%P/S

配套培养基货号：TCH-G365L

传代比例：1:3-1:6，每2-3天换液一次

倍增时间：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590

Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487

Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590"

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