HyCyte® | 海星生物

产品名称：B16-F10细胞Luc&tdTomato基因过表达稳转细胞株

产品货号：TCM-C713LR

转导方法：慢病毒法

抗性基因：Puro

荧光基因：Luc&tdTomato

克隆类型：Poolcells

靶细胞名称：小鼠皮肤黑色素瘤细胞

靶细胞简称：B16-F10

种属来源：小鼠

组织来源：皮肤

疾病特征：黑色素瘤

细胞形态：上皮细胞样混有梭形细胞

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS（胎牛血清）+1.0 ug/mL Puromycin+1%P/S

配套培养基货号：TCM-G713LR

传代比例：1:3-1:6，每2-3天换液一次

倍增时间：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790

Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987

Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034

Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927

Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183

Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654

Li M, et al. Loss of intracisternal A-type retroviral particles in BL6 melanoma cells transfected with MHC class I genes. J.Gen. Virol. 77: 2757-2765, 1996. PubMed: 8922469

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