产品名称:PANC-1细胞hCASK基因敲除株(CGKO-M3341)
产品简称:PANC-1 KO hGLYR1
产品货号:CGKO-M3341
修饰基因:hCASK
基因ID:8573
修饰类型:敲除
转导方法:蛋白法
抗性基因:—
克隆类型:纯合子
细胞鉴定:PCR,测序
种属来源:人
组织来源:胰腺、胰管
疾病特征:上皮细胞癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:DMEM-H+10%FBS+1%P/S
配套培养基货号:TCH-G296
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870
Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626
Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903