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产品名称：293T细胞hMECP2基因敲除株（CGKO-M3455）
产品简称：HEK293T KO-hMECP2
产品货号：CGKO-M3455
修饰基因：hMECP2
基因ID：4204
修饰类型：敲除
转导方法：蛋白法
抗性基因：—
克隆类型：纯合子
细胞鉴定：PCR，测序，WB
种属来源：人
组织来源：胚肾
疾病特征：—
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系：DMEM-H+10%FBS+1%P/S
配套培养基货号：TCH-G101
传代比例：1:4-1:8，每2-3天换液一次
传代周期：24-48 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed: 32094589
Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361
Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999
Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001
Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960