- 产品参数
- 产品详情
- 说明书
- 相关产品
产品名称：BV2细胞mGpnmb基因敲除株（CGKO-M3499）
产品简称：BV-2 KO-mGpnmb
产品货号：CGKO-M3499
修饰基因：mGpnmb
基因ID：93695
修饰类型：敲除
转导方法：蛋白法
抗性基因：—
克隆类型：纯合子
细胞鉴定：PCR，测序
种属来源：小鼠
组织来源：脑
疾病特征：/
细胞形态：多形型
生长特性：半贴壁生长
培养体系：DMEM-H+10%FBS+1%P/S
配套培养基货号：TCM-G718
传代比例：1:3-1:6，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
"Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.
In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
Proteomics 15:3617-3622(2015)
Stansley B., Post J., Hensley K.
A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.
J. Neuroinflamm. 9:115-115(2012)
Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.
BMC Genomics 12:14-14(2011)
Crain J.M., Watters J.J.
Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.
Open Drug Discov. J. 2:148-167(2010)
Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.
The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.
ALTEX 26:83-94(2009)"