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产品名称：BV2细胞mSelenop基因过表达稳转细胞株（CGSC-M32761）
产品货号：CGSC-M32761
基因名称：mSelenop[NM_009155.4，1143 bp]
基因ID：20363
转导方法：转座子
转录本：NM_009155.4
抗性基因：Puro
荧光基因：EGFP
验证方式：qPCR
克隆类型：Poolcells
靶细胞名称：小鼠小胶质细胞
靶细胞简称：BV2
种属来源：小鼠
组织来源：脑
疾病特征：/
细胞形态：多形型
生长特性：半贴壁生长
培养体系：DMEM-H+10%FBS+1%P/S
配套培养基货号：TCM-G718
传代比例：1:3-1:6，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
"Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.
In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
Proteomics 15:3617-3622(2015)
Stansley B., Post J., Hensley K.
A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.
J. Neuroinflamm. 9:115-115(2012)
Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.
BMC Genomics 12:14-14(2011)
Crain J.M., Watters J.J.
Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.
Open Drug Discov. J. 2:148-167(2010)
Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.
The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.
ALTEX 26:83-94(2009)"