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产品名称：CHO-K1（贴壁）细胞hCALCR基因过表达稳转细胞株（CGSC-M33131）
产品货号：CGSC-M33131
基因名称：hCALCR[NM_001164737.3，1743 bp]
基因ID：799
转导方法：转座子法
转录本：NM_001164737.3
抗性基因：Puro
荧光基因：EGFP
验证方式：qPCR
克隆类型：poolcells
靶细胞名称：仓鼠卵巢细胞
靶细胞简称：CHO-K1
种属来源：仓鼠
组织来源：卵巢
疾病特征：—
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系：F12K+10%FBS+1%P/S
配套培养基货号：TCO-G005
传代比例：1:3-1:6，每2-3天换液一次
倍增时间：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821
Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058
Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736
Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403
Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351
Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752
Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365