产品名称:NCI-H1299细胞hSLC16A1基因过表达稳转细胞株(CGSC-M33621)
产品货号:CGSC-M33621
基因名称:hSLC16A1[NM_001166496.2,1503 bp]
基因ID:6566
转导方法:转座子法
转录本:NM_001166496.2
抗性基因:Puro
荧光基因:EGFP
验证方式:qPCR
克隆类型:Poolcells
种属来源:人
组织来源:肺
疾病特征:非小细胞肺癌
细胞形态:上皮样/多角形
生长特性:贴壁生长
培养体系:RPMI-1640+10%FBS+1%P/S
配套培养基货号:TCH-G269
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.