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产品名称：MIA PaCa-2细胞hABHD17C[NM_021214.2，990 bp]基因过表达稳转细胞株（CGSC-M34931）
产品货号：CGSC-M34931
基因名称：hABHD17C[NM_021214.2，990 bp]
基因ID：58489
转导方法：转座子法
转录本：NM_021214.2
抗性基因：Puro
荧光基因：EGFP
验证方式：qPCR
克隆类型：Poolcells
种属来源：人
组织来源：胰腺
疾病特征：胰腺癌
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系：DMEM-H+10%FBS+2.5%马血清+1% Sodium Pyruvate+1%P/S
配套培养基货号：TCH-G259
传代比例：1:3-1:6，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918