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产品名称：PC12(未分化)细胞rNlgn1基因过表达稳转细胞株（CGSC-M35101）
产品货号：CGSC-M36001
基因名称：rNlgn1
基因ID：116647
转导方法：转座子法
转录本：NM_053868.2
抗性基因：Puro
荧光基因：——
验证方式：qPCR
克隆类型：Poolcells
种属来源：大鼠
组织来源：肾上腺
疾病特征：嗜铬细胞瘤
细胞形态：小的形状不规则细胞
生长特性：悬浮成簇生长、伴有少量松散贴壁细胞
培养体系：RPMI-1640+5%FBS（胎牛血清）+10%HS（马血清）+1%P/S
配套培养基货号：TCR-G617
传代比例：1:3-1:6，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712
Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125
U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897