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产品名称：HuH-7细胞Cas9基因过表达稳转株
产品货号：TCH-C217C
转导方法：慢病毒
抗性基因：Hygro
荧光基因：/
验证方式：qPCR
克隆类型：poolcells&单克隆
种属来源：人
组织来源：肝脏
疾病特征：肝癌
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系：DMEM-H+10%FBS（胎牛血清）+1%Glutamax+1% Sodium Pyruvate+200.0 ug/mL Hygromycin+1%P/S
配套培养基货号：TCH-G217C
传代比例：1:2-1:3，每2-3天换液一次
倍增时间：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
参考文献：
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)
Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)