产品名称:C1498细胞GFP&LUC基因过表达稳转细胞株
产品货号:TCM-C785LG
转导方法:慢病毒
抗性基因:Puro
荧光基因:GFP&LUC
验证方式:qPCR
克隆类型:Poolcells
种属来源:小鼠
组织来源:淋巴母细胞
疾病特征:淋巴细胞癌
细胞形态:淋巴母细胞
生长特性:悬浮生长
培养体系:DMEM-H+10%FBS(胎牛血清)+4.0 ug/mL Puromycin+1%P/S
配套培养基货号:TCM-G785LG
传代比例:1:3-1:6,每2-3天换液一次
倍增时间:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
Boyer MW, et al. Thr role of B7 costimulation by murine acute myeloid leukemia in the generation and function of a CD8+ T-cell line with potent in vivo graft-versus-leukemia properties. Blood 89: 3477-3485, 1997. PubMed: 9129056