HyCyte® | 海星生物

产品名称：NIH/3T3细胞cas9基因过表达稳转细胞株

产品货号：TCM-C752C

转导方法：慢病毒

抗性基因：Hygro

荧光基因：/

验证方式：qPCR

克隆类型：Poolcells

种属来源：小鼠

组织来源：胚胎

疾病特征：/

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%CS（新生牛血清）+1600.0 ug/mL Hygromycin+1%P/S

配套培养基货号：TCM-G752C

传代比例：1:3-1:6，每2-3天换液一次

倍增时间：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

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Andersson P, et al. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell 16: 63-75, 1979. PubMed: 84715

Copeland NG, Cooper GM. Transfection by exogenous and endogenous murine retrovirus DNAs. Cell 16: 347-356, 1979. PubMed: 222457

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Jones PL, et al. Tumor necrosis factor alpha and interleukin-1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-beta and NF-kappaB. Mol. Cell. Biol. 17: 6970-6981, 1997. PubMed: 9372929

Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915