Positive WB detected in:A431 cells, HepG2 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected in:——
Positive IHC detected in:mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in:HeLa cells, HepG2 cells
Positive FC (Intra) detected in:——
推荐稀释比:应用 推荐稀释比
Western Blot (WB) WB : 1:5000-1:50000
Immunohistochemistry (IHC) IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原:Domain: 1151-1350 aa of NM_003566
Sequence: KLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWA
抗体类别:Monoclonal
产品类型:Antibody
全称:early endosome antigen 1
计算分子量:162 kDa
观测分子量:162 kDa
GenBank蛋白编号:NM_003566
基因名称:EEA1
Gene ID (NCBI):8411
偶联类型:Unconjugated
形式:Liquid
纯化方式:Protein G purification
UNIPROT ID:«UNIPROT ID»
储存缓冲液:PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件:Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍:Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。