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Positive WB detected in：HEK-293T cells, HeLa cells, PC-3 cells, HepG2 cells
Positive IP detected in：HEK-293T cells
Positive IHC detected in：mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in：HeLa cells
Positive FC (Intra) detected in：——
推荐稀释比：应用 推荐稀释比
Western Blot (WB) WB : 1:5000-1:50000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原：Domain: 254-390 aa of BC068005
Sequence: CDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKE
抗体类别：Polyclonal
产品类型：Antibody
全称：ligase III, DNA, ATP-dependent
计算分子量：100-110 kDa
观测分子量：100 kDa
GenBank蛋白编号：BC068005
基因名称：LIG3
Gene ID (NCBI)：3980
偶联类型：Unconjugated
形式：Liquid
纯化方式：Antigen affinity purification
UNIPROT ID：«UNIPROT ID»
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍：DNA ligase III(DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。