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Positive WB detected in：LNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected in：——
Positive IHC detected in：mouse testis tissue, human lung cancer tissue, rat testis tissue, human oesophagus cancer tissue, human colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed
Positive IF/ICC detected in：MCF-7 cells
Positive FC (Intra) detected in：——
推荐稀释比：应用 推荐稀释比
Western Blot (WB) WB : 1:5000-1:50000
Immunohistochemistry (IHC) IHC : 1:200-1:1000
Immunofluorescence (IF)-P IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原：Domain: 229-580 aa of BC001650
Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL
抗体类别：Recombinant
产品类型：Antibody
全称：methyltransferase like 3
计算分子量：64 kDa
观测分子量：65-70 kDa
GenBank蛋白编号：BC001650
基因名称：METTL3
Gene ID (NCBI)：56339
偶联类型：Unconjugated
形式：Liquid
纯化方式：Protein A purification
UNIPROT ID：«UNIPROT ID»
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍：METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。