Positive WB detected in:HeLa cells, A549 cells, mouse skin tissue, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected in:HEK-293 cells
Positive IHC detected in:human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in:——
Positive FC (Intra) detected in:——
推荐稀释比:应用 推荐稀释比
Western Blot (WB) WB : 1:1000-1:4000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原:Domain: 226-332 aa of BC108925
Sequence: PLAATPLGNGLGGLHNAQQSLPNYGPPGHDKMLSPNFDAHHTAMLTRGEQHLSRGLGTPPAAMMSHLNGLHHPGHTQSHGPVLAPSRERPPSSSSGSQVATSGQLEE
抗体类别:Polyclonal
产品类型:Antibody
全称:one cut homeobox 2
计算分子量:504 aa, 54 kDa
观测分子量:54-60 kDa
GenBank蛋白编号:BC108925
基因名称:ONECUT2
Gene ID (NCBI):9480
偶联类型:Unconjugated
形式:Liquid
纯化方式:Antigen affinity purification
UNIPROT ID:«UNIPROT ID»
储存缓冲液:PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件:Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍:ONECUT2, also named as One cut Domain family member 2 or Hepatocyte nuclear factor 6-beta, is a 504 amino acid protein, which contains one CUT DNA-binding Domain and one homeobox DNA-binding Domain. ONECUT2 belongs to the CUT homeobox family and activates the transcription of a number of liver genes such as HNF3B. ONECUT2 is required for liver differnetiation and metabolism. ONECUT2 and ONECUT3 play redundant roles with ONECUT1 in pancreas development.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
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