HyCyte® | 海星生物

Positive WB detected in：HEK-293 cells, A549 cells, HeLa cells

Positive IP detected in：——

Positive IHC detected in：mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in：——

Positive FC (Intra) detected in：——

推荐稀释比：应用 推荐稀释比

Western Blot (WB) WB : 1:2000-1:12000

Immunohistochemistry (IHC) IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

文献引用反应性：

免疫原：CatNo: Ag30152

Product name: Recombinant human rPTPσ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 825-1000 aa of NM_002850

Sequence: VVVTKGAVLGRPTLSVQQTPEGSLLARWEPPAGTAEDQVLGYRLQFGREDSTPLATLEFPPSEDRYTASGVHKGATYVFRLAARSRGGLGEEAAEVLSIPEDTPRGHPQILEAAGNASAGTVLLRWLPPVPAERNGAIVKYTVAVREAGALGPARETELPAAAEPGAENALTLQGL

抗体类别：Polyclonal

产品类型：Antibody

全称：protein tyrosine phosphatase, receptor type, S

计算分子量：217 kDa

观测分子量：140 kDa

GenBank蛋白编号：NM_002850

基因名称：PTPRS

Gene ID (NCBI)：5802

RRID：AB_2918301

偶联类型：Unconjugated

形式：Liquid

纯化方式：Antigen affinity purification

UNIPROT ID：Q13332

储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍：PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa.

注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。