HyCyte® | 海星生物

Positive WB detected in：Human peripheral blood platelets

Positive IP detected in：——

Positive IHC detected in：human spleen tissue, human liver tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF/ICC detected in：——

Positive FC (Intra) detected in：——

推荐稀释比：应用 推荐稀释比

Western Blot (WB) WB : 1:500-1:2000

Immunohistochemistry (IHC) IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent.

免疫原：Peptide

抗体类别：Polyclonal

产品类型：Antibody

全称：CD36 molecule (thrombospondin receptor)

计算分子量：472 aa, 53 kDa

观测分子量：88 kDa

GenBank蛋白编号：BC008406

基因名称：CD36

Gene ID (NCBI)：948

偶联类型：Unconjugated

形式：Liquid

纯化方式：Antigen affinity purification

UNIPROT ID：«UNIPROT ID»

储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

背景介绍：Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

Polymorphisms in CD36 can increase susceptibility to malaria.

AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular Domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane Domains can be phosphorylated and play a role in signal transduction. Intracellular Domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.

注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。