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Positive WB detected in：HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, NIH/3T3 cells, rat liver tissue, HEK-293 cells, HepG2 cells, C2C12 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in：——
Positive IHC detected in：human liver tissue, human breast cancer tissue, human colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in：——
Positive FC (Intra) detected in：HepG2 cells
推荐稀释比：应用 推荐稀释比
Western Blot (WB) WB : 1:1000-1:8000
Immunohistochemistry (IHC) IHC : 1:500-1:2000
Immunofluorescence (IF)-P IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA) FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent.
免疫原：Domain: 1-105 aa of BC009578
Sequence: MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE
抗体类别：Polyclonal
产品类型：Antibody
全称：cytochrome c, somatic
计算分子量：12 kDa
观测分子量：12-15 kDa
GenBank蛋白编号：BC009578
基因名称：Cytochrome c
Gene ID (NCBI)：54205
偶联类型：Unconjugated
形式：Liquid
纯化方式：Antigen affinity purification
UNIPROT ID：«UNIPROT ID»
储存缓冲液：PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件：Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
背景介绍：Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c ca99n be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
注意事项
1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗等用途。
2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。